Toxoplasmosis is a parasitic disease, caused by Toxoplasma gondii. This parasite infects hot blood creatures like human .This infection is associated with congenital infections, abortion, hydrocephalus or systemic infection in immunosuppressed patients, especially with those who acquired immunodeficiency syndrome (AIDS). In this research we studied the prevalence of toxoplasmosis in Lattakia city and its country in the several levels of the society , besides of the affection of sex and environment on the infection. We compared between ELISA and Latex to determine how much latex can be useful, taking in consideration that Latex is a simple and inexpensive method . we found a wide spread levels of infection, and low knowledge about toxoplasmosis.